Issue link: https://ahint.epubxp.com/i/574474
30 ASHI Quarterly Third Quarter 2015 Development and Validation of a Rapid Optimized Flow Cytometry Crossmatch (FCXM) Assay, the Halifax and Halifaster FCXM Protocols 1 Flow cytometry crossmatch sensitivity can be increased by: A Increasing the temperature of incubation from 4 o C to RT B Decreasing incubation time with serum C Decreasing serum volume D All of the above 2 Treatment of target cells with pronase reduces FC receptor expression and decreases B cell background in FCXM A True B False 3 The sensitivity of the flow cytometry crossmatch assay can be increased by decreasing the number of target cells per reaction A True B False 4 DNase treatment is used to eliminate dead and dying cells A True B False 5 Which of the following parameters were used to expedite the flow cytometric crossmatch assay: A Changing the crossmatch platform from tube method to 96-well tray method B Shortening of centrifugation time during wash steps C Shortening of incubation time with serum D Shortening of incubation time with anti-IgG-FITC E All of the above Significance of Complement Fixing Antibody Testing in Kidney Transplantation 6 Classical pathway is activated by A Sugar residue like mannose binding lectin B Microbial components C Specific antibodies 7 C1q assay detects A Initial step of complement activation by the Classical pathway B Downstream step of complement activation by the Alternative pathway C End product of the complement activation by the Classical pathway D None of the above 8 CDC testing detects A Final step of the complement activation by the Classical pathway only B Dead cells caused by MAC formation on the plasma membrane C Final step of the complement activation by all 3 pathways D "A" and "B" E "B" and "C" 9 Flow Cytometry Crossmatch cannot distinguish complement fixing antibodies from complement non-fixing antibodies A True B False 10 What is the MAC? A Membrane Attack Complex, the terminal component of the Classical Pathway of complement activation, which makes holes on the plasma membrane B Membrane Attack Complex, the terminal component of all three complement activation pathways, which lyses the plasma membrane C Membrane Attack Complex, the middle component of the complement cascade, where all three pathways merge ASHI Quarterly Continuing Education Quiz Quiz Instructions: The multiple choice quiz below is provided as an alternative method of earning CE credit hours. Read each article then select the ONE best answer to each question. The questions are based solely on the content of the article. Answer all of the questions, and send the quiz answer sheet and fee (if applicable) to the ASHI Executive Office before the processing date listed on the answer sheet. To be eligible for 3.0 credit/contact hours or 0.45 Continuing Education Credits (CECs), ALL questions must be answered, a passing score of 70% must be obtained and the answer sheet must be submitted with fee (if applicable) before the deadline. Quizzes may not be retaken, nor can ASHI staff respond to questions. Allow six weeks for paper processing following the submission deadline to receive return notification of your completion of the CE process. The CE credit will be dated when it is submitted for grading. That date will determine the CE cycle year. Quiz may be taken online at www.ashi-u.com.